tslp elisa development kit Search Results


tslp  (R&D Systems)
94
R&D Systems tslp
Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. ( a ) IL-1α ( b ) IL-1β and ( c ) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of ( d ) IL-1α & ( e ) IL-1β released from PAECs was measured by <t>ELISA.</t> Means ± SEM are shown. Exact P values indicated.
Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tslp/product/R&D Systems
Average 94 stars, based on 1 article reviews
tslp - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
tslp elisa development kit  (PeproTech)
90
PeproTech tslp elisa development kit
Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. ( a ) IL-1α ( b ) IL-1β and ( c ) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of ( d ) IL-1α & ( e ) IL-1β released from PAECs was measured by <t>ELISA.</t> Means ± SEM are shown. Exact P values indicated.
Tslp Elisa Development Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tslp elisa development kit/product/PeproTech
Average 90 stars, based on 1 article reviews
tslp elisa development kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. ( a ) IL-1α ( b ) IL-1β and ( c ) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of ( d ) IL-1α & ( e ) IL-1β released from PAECs was measured by ELISA. Means ± SEM are shown. Exact P values indicated.

Journal: Scientific Reports

Article Title: Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma

doi: 10.1038/s41598-020-65567-z

Figure Lengend Snippet: Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. ( a ) IL-1α ( b ) IL-1β and ( c ) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of ( d ) IL-1α & ( e ) IL-1β released from PAECs was measured by ELISA. Means ± SEM are shown. Exact P values indicated.

Article Snippet: Cell-culture supernatants were also harvested on the same days and samples were assessed in duplicate via the ELISA kits for IL-1α, IL-1β, IL-33, IL-6, IL-8, GM-CSF and TSLP (R&D Systems, Minneapolis) according to manufacturer instructions and the concentration (pg/ml) normalized to total protein content (pg/mg of total protein) assessed using the Pierce Rapid Gold BCA Protein Assay Kit (Thermofisher).

Techniques: Cell Culture, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay

IL-1 but not IL-33 stimulates the release of inflammatory mediators from primary airway fibroblasts (PAFs). Primary airway fibroblasts from non-asthmatics (n = 5–9) and asthmatics (n = 5–9) were grown to confluence on collagen I coated plates and stimulated with or without 1 ng/ml recombinant human IL-1α, IL-1β or IL-33 for 24 hours. Concentration of ( a ) IL-6, ( b ) CXCL8/IL-8, ( c ) granulocyte-monocyte colony stimulating factor (GM-CSF) & ( d ) thymic stromal lymphopoietin (TSLP) released from primary airway fibroblasts after 24 hours. Exact P values indicated.

Journal: Scientific Reports

Article Title: Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma

doi: 10.1038/s41598-020-65567-z

Figure Lengend Snippet: IL-1 but not IL-33 stimulates the release of inflammatory mediators from primary airway fibroblasts (PAFs). Primary airway fibroblasts from non-asthmatics (n = 5–9) and asthmatics (n = 5–9) were grown to confluence on collagen I coated plates and stimulated with or without 1 ng/ml recombinant human IL-1α, IL-1β or IL-33 for 24 hours. Concentration of ( a ) IL-6, ( b ) CXCL8/IL-8, ( c ) granulocyte-monocyte colony stimulating factor (GM-CSF) & ( d ) thymic stromal lymphopoietin (TSLP) released from primary airway fibroblasts after 24 hours. Exact P values indicated.

Article Snippet: Cell-culture supernatants were also harvested on the same days and samples were assessed in duplicate via the ELISA kits for IL-1α, IL-1β, IL-33, IL-6, IL-8, GM-CSF and TSLP (R&D Systems, Minneapolis) according to manufacturer instructions and the concentration (pg/ml) normalized to total protein content (pg/mg of total protein) assessed using the Pierce Rapid Gold BCA Protein Assay Kit (Thermofisher).

Techniques: Recombinant, Concentration Assay